Oscope: a statistical pipeline for identifying oscillatory genes in unsynchronized single cell RNA-seq experiments
نویسندگان
چکیده
2 Run Oscope 3 2.1 Required inputs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 2.2 Normalization . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 2.3 Pre-processing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 2.4 Rescaling . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 2.5 Oscope: paired-sine model . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 2.6 Oscope: K-medoids algorithm . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 2.7 Flag clusters with small within-cluster sine scores and/or small within-cluster phase shifts . . . . . . . . . 6 2.8 Oscope: extended nearest insertion . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
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